Journal article
γδ T cells mediate robust anti-HIV functions during antiretroviral therapy regardless of immune checkpoint expression
KR Field, KM Wragg, SJ Kent, WS Lee, JA Juno
Clinical and Translational Immunology | Published : 2024
DOI: 10.1002/cti2.1486
Abstract
Objectives: Although antiretroviral therapy (ART) efficiently suppresses HIV viral load, immune dysregulation and dysfunction persist in people living with HIV (PLWH). γδ T cells are functionally impaired during untreated HIV infection, but the extent to which they are reconstituted upon ART is currently unclear. Methods: Utilising a cohort of ART-treated PLWH, we assessed the frequency and phenotype, characterised in vitro functional responses and defined the impact of immune checkpoint marker expression on effector functions of both Vδ1 and Vδ2 T cells. We additionally explore the in vitro expansion of Vδ2 T cells from PLWH on ART and the mechanisms by which such expanded cells may sense a..
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Awarded by National Institutes of Health
Funding Acknowledgements
We are grateful to both healthy volunteers and clinical subjects for their generous donation of samples for this study. We thank J Silvers, H Kent, A Kristensen, A Wheatley, M Parsons and the physicians at the Melbourne Sexual Health Centre for help recruiting subjects. We are grateful to C Batten, T Amarasena and J Rambhatla for technical assistance. We acknowledge the Melbourne Cytometry Platform for provision of flow cytometry services. The following reagents were obtained through the NIH HIV Reagent Program, Division of AIDS, NIAID, NIH: human immunodeficiency virus 1 (HIV-1) lymphadenopathy-associated virus (LAV)-infected 8E5 cells, ARP-95, contributed by Dr Thomas Folks; EGFP-CEMNKr cells, ARP-11698, contributed by Dr Wannee Kantakamalakul; CEM.NKR CCR5+ cells, ARP-4376, contributed by Dr Alexandra Trkola. JAJ, SJK and WSL are supported by NHMRC investigator grants and the project was funded by an NHMRC Program Grant (1149990). Open access publishing facilitated by The University of Melbourne, as part of the Wiley - The University of Melbourne agreement via the Council of Australian University Librarians.